Manchester United are aiming to claim a fourth domestic Double having won the Premiership title ahead of Chelsea.
Chelsea were the last club to win the FA Cup final at the old Wembley in 2000 and they are determined to end United's double dreams.
The FA Cup is the only domestic trophy Jose Mourinho has failed to win so far during his time at Chelsea and he will be hoping to add it to their Carling Cup success from earlier this season.
This season's two meetings between the giants ended in draws, but Mourinho boasts a good record against Sir Alex Ferguson having lost just one of eight clashes against the Scot.
Chelsea go into the game with a crippling injury list with Mourinho claiming he only had 15 players available to pick from.
Ricardo Carvalho, Michael Ballack and Andrei Shevchenko have all been ruled out through injury.
In the absence of Carvalho, Michael Essien is set to start in central defence alongside Blues skipper John Terry.
Arjen Robben is expected to be on the bench after recovering from a knee injury.
Ashley Cole will be given until the last minute to prove his fitness as he struggles with an ankle injury with Wayne Bridge standing by to replace him.
John Obi Mikel is set to undergo a late fitness test as he looks to shake off a thigh problem.
Manchester United are missing Gary Neville and Louis Saha for the trip to Wembley.
Wes Brown and John O'Shea are both battling it out to take Neville's place at right-back.
Ryan Giggs will take over the captain's armband from Neville as he looks to win his fifth FA Cup winners' medal.
Ferguson also has a selection dilemma at left-back with Patrice Evra and Gabriel Heinze vying for the spot.
United chief Ferguson must also decide what formation to play as he could decide to bolster his midfield with the inclusion of Darren Fletcher or opt to give Alan Smith a start in attack.
United's Player of the Season Cristiano Ronaldo is in line to make his 150th start for the club against Chelsea.